I am Anirudh. A PhD student at the University of Sheffield under the MRC funded DiMeN DTP programme working on nature's own killing machine - bacteriophages.
I work with infection causing C. difficile bacteria and their bacteriophages and trying to understand how they work and "upgrade" them for therapeutic use.
Host interaction study using phages. Testing the host range of experimentally evolved phages, and engineering phage genome.
Interested in studying how microbe-host interactions and phages be used for assisting AMRs treatments, and increase the host range of phages.
Cloning, mutation, and imaging of ParM and MreB proteins, and their light microscopy using DeltaVision and Leica. Further analysis is done using ImageJ.
Dr. Srinivasan's lab is working on analysis of ParM homologs. ParM plays a vital role in the plasmid segregation process of bacteria. It helps the bacteria to maintain antibiotic resistant genes with plasmids necessary for it's survival. We visualized various ParM proteins after expressing them in a heterologous system. The project will further explore the dynamic properties of the filaments formed by ParM and how it varies between different homologs.
Dr. Srinivasan's lab is also working on MreB studies, a cytoskeletal protein that helps preserve cell shape. I contributed to the project by mutating MreB in specific regions and checking the effect on polymerization using a heterologous system of Schizosaccharomyces pombe
Dr. Bertels' lab is using directed evolution experiments to explore host recognition by bacteriophages. I contributed to establish a standard procedure to carry out phage genome mutagenesis and analyzed the host range of experimentally evolved mutant phages.
Clostridiodes difficile is one of the most common hospital borne pathogen, notorious for recurrence of infection in antibiotics treatment. At the University of Sheffield, we are engineering bacteriophages with various structural and functional mutations to understand C. difficile phage infections. We are also generating phage mutants with extra additions which will allow us to use this phage as a tool to target infection causing C. difficile bacteria found in human gut.
Mishra D, Jakhmola A, Srinivasan R. A role for the last C-terminal helix of the F plasmid segregating protein SopA in nucleoid binding and plasmid maintenance. Plasmid. 2022 Jan 1;119:102617.
Currently based in Sheffield, UK.
Feel free to send me papers, ask project questions, and discuss technology.
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Apart from my academics, I am experienced and interested in technology, no-coding alternatives, automation, artificial intelligence, and various tools like Notion.
